The Niquet stream biodiversity reserve is located in the Nord-du-Québec administrative region, between 50°04’ and 50°14’ north latitude and 78°53’ and 79°10’ west longitude. It is situated 93 km north-west of Matagami and 110 km south of the Cree community of Waskaganish. The proposed biodiversity reserve covers an area of 164.6 km² and lies within the Baie-James Municipality.